LOCUS       ISCR1-bleMBL-blaNDM-3-arr-3_unit5084 bp   DNA linear
BCT26-OCT-2020
DEFINITION  Klebsiella pneumoniae.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella
            pneumoniae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR1-bleMBL-blaNDM-3-arr-3 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
     mobile_element  1..5084
                     /gene="ISCR1-bleMBL-blaNDM-3-arr-3 unit"
                     /product="Putative resistance unit:
                     ISCR1-bleMBL-blaNDM-3-arr-3 unit"
     mobile_element  complement(1..2154)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             381..1922
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     misc_recomb     2134..2154
                     /gene="oriIS"
                     /product="ISCR1 oriIS"
     mobile_element  complement(2155..4024)
                     /gene="truncated Tn125"
                     /product="Composite transposon: truncated Tn125"
     misc_feature    complement(2155..2594)
                     /gene="truncated trpF"
                     /product="Truncated N-(5'-phosphoribosyl)anthranilate
                     isomerase (pseudogene)"
     CDS             complement(2599..2964)
                     /gene="bleMBL"
                     /codon_start=1
                     /transl_table=11
                     /product="Bleomycin resistance protein"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     CDS             complement(2968..3780)
                     /gene="blaNDM-3"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase NDM-3"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTNDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     mobile_element  complement(3874..4024)
                     /gene="truncated ISAba125"
                     /product="Insertion sequence: truncated ISAba125"
     repeat_region   complement(3874..3899)
                     /gene="IRR_ISAba125"
                     /product="ISAba125 inverted repeat right"
     misc_feature    complement(3881..4024)
                     /gene="truncated tnpA"
                     /product="Truncated ISAba125 transposase"
     misc_feature    4023..4436
                     /gene="truncated catB3"
                     /product="Truncated Chloramphenicol acetyltransferase"
     CDS             4521..4973
                     /gene="arr-3"
                     /codon_start=1
                     /transl_table=11
                     /product="Rifampin ADP-ribosyltransferase"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFED
                     GRILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKRFPGN
                     PTQSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
BASE COUNT     1203 a   1411 c   1371 g   1099 t
ORIGIN
        1 aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa
       61 agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag
      121 ctcgacgact accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat
      181 aacagttggg gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt
      241 ctcgctcaac cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta
      301 gcgatgtcta gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac
      361 agtatcagcg gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca
      421 ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg
      481 agcagcacta cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct
      541 acatccaaca agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc
      601 gggttcgctg cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg
      661 gcttttgccc tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg
      721 aagtcttccc caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct
      781 ttttgctggc tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac
      841 tctcaactca tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag
      901 tgactcttat ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt
      961 ttctcgatgg tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg
     1021 cacccactta cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct
     1081 gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag
     1141 agggcgaaga cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg
     1201 tcggccccca gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata
     1261 aagccgactc aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga
     1321 tggccgaagc gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag
     1381 cggtttcaga aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa
     1441 ctccgtaccg caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac
     1501 tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac
     1561 cgaacagcaa acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat
     1621 cggaaggtct cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga
     1681 cctggatgca acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg
     1741 gcggtcacgt caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca
     1801 agcatctgaa acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag
     1861 aacgagcgcc gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact
     1921 gacgacccca aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag
     1981 tttgtatctc tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc
     2041 ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt
     2101 caggtaagat acttcggatg aggagcaaaa aggtggttta tacttcctat acccaacgcc
     2161 aaccccatgc cgccgggcag cgcgcctttg ggggtcttgg cgtcgaacaa gatcaagtcc
     2221 gccgccccgg cataggctgc ggcgcgtgcg acatcgctgg cgctggcgac gggcagcgcc
     2281 ttccacaccg gcttgccaaa ccgcgcgcgc aactgggcca cgcgttcggg cgattccgaa
     2341 ccgtgcagct gcagcgcgtt cagcttggct gccaccagtg cgtcggcgat gacagcatca
     2401 tccgcatcga cgaacaaacc gaccatggcg atctggccag ctgcgcgcga tgtcaaagcg
     2461 cccgcgacat tcgacgtaac cgcacggggc gacgctggat agaacaccaa cccggcatag
     2521 tccgcccgcg ccgcgatggt cgcatcgagc gcctcgggtg tgctgatccc gcaaatcttg
     2581 attttcgcgg gcatgcggtc agtcggggtt ctggatcagc cgcaccagcg tgcagtcggg
     2641 atcgatcagg tagccgatcc tcaggccgct cgcctccagt tgcggagctt tgaagcgcgg
     2701 ccagccggtg cttttttcct cggctcccgc cgcgttcacc aatgccacca tggcatcgag
     2761 atcatccaac cgcaggcaac agccgaacga gctcgtagct gggtcgaggt caggataggg
     2821 gaagaattcg agctgcaaac cgccgcgctg caggatcatc cagccgcgat ccttccaact
     2881 cgtcgcaaag cccagcttcg cataaaacgc ctctgtcaca tcgaaatcgc gcgatggcag
     2941 attgggggtg acgtggtcag ccatggctca gcgcagcttg tcggccatgc gggccgtatg
     3001 agtgattgcg gcgcggctat cgggggcgga atggctcatc acgatcatgc tggccttggg
     3061 gaacgccgca ccaaacgcgc gcgctgacgc ggcgtagtgc tcagtgtcgg catcaccgag
     3121 attgccgagc gacttggcct tgctgtcctt gatcaggcag ccaccaaaag cgatgtcggt
     3181 gccgtcgatc ccaacggtga tattgtcact ggtgtggccg gggccggggt aaaatacctt
     3241 gagcgggcca aagttgggcg cggttgctgg ttcgacccag ccattggcgg cgaaagtcag
     3301 gctgtgttgc gccgcaacca tcccctcttg cggggcaagc tggttcgaca acgcattggc
     3361 ataagtcgca atccccgccg catgcagcgc gtccataccg cccatcttgt cctgatgcgc
     3421 gtgagtcacc accgccagcg cgaccggcag gttgatctcc tgcttgatcc agttgaggat
     3481 ctgggcggtc tggtcattgg tccaggcggt atcgaccacc agcacgcggc cgccatccct
     3541 gacgatcaaa ccgttggaag cgactgcccc gaaacccggc atgtcgagat aggaagtgtg
     3601 ctgccagaca ttcggtgcga gctggcggaa aaccagatcg ccaaaccgtt ggtcgccagt
     3661 ttccatttgc tggccaatcg tcgggcggat ttcaccgggc atgcacccgc tcagcatcaa
     3721 tgcagcggct aatgcggtgc tcagcttcgc gaccgggtgc ataatattgg gcaattccat
     3781 caagttttcc ttttattcag cattaaaaac cccgcaaatg cgaggcctag taaatagatg
     3841 atcttaattt ggttcactgt agcaaaaata tggggcgaat tcaaacatga ggtgcgacag
     3901 tttcaaaagc catatgataa tcaacaagct gagcaaattt ctctaatggt gtaagccaat
     3961 ctaacgcctt tctaggacga gtattcagtg acatggcaac ttgatttaaa taatgctgat
     4021 ctgctggcaa tcaggggcat cggtacgact gggcatcatc tttcccgttc ttttatatgc
     4081 aggaagaacc tgcattctca agcgcactcg atgccttcca aaaagcaggt aatactgtca
     4141 ttggcaatga cgtttggatc ggctctgagg caatggtcat gcccggaatc aagatcgggc
     4201 acggtgcggt gataggcagc cgctcgttgg tgacaaaaga tgtggagcct tacgctatcg
     4261 ttggcggcaa tcccgctaag aagattaaga aacgcttcac cgatgaggaa atttcattgc
     4321 ttctggagat ggagtggtgg aattggtcac tggagaagat caaagcggca atgcccatgc
     4381 tgtgctcgtc taatattgtt ggcctgcaca agtattggct cgagtttgcc gtctaacaat
     4441 tcaatcaagc cgatgccgct tcgcggcacg gcttatttca ggcgttatgc agccaaatcc
     4501 caacaattaa gggtcttaaa atggtaaaag attggattcc catctctcat gataattaca
     4561 agcaggtgca aggaccgttc tatcatggaa ccaaagccaa tttggcgatt ggtgacttgc
     4621 taaccacagg gttcatctct catttcgagg acggtcgtat tcttaagcac atctactttt
     4681 cagccttgat ggagccagca gtttggggag ctgaacttgc tatgtcactg tctggcctcg
     4741 agggtcgcgg ctacatatac atagttgagc caacaggacc gttcgaagac gatccgaatc
     4801 ttacgaacaa aagatttccc ggtaatccaa cacagtccta tagaacctgc gaacccttga
     4861 gaattgttgg cgttgttgaa gactgggagg ggcatcctgt tgaattaata aggggaatgt
     4921 tggattcgtt ggaggactta aagcgccgtg gtttacacgt cattgaagac tagtcctttg
     4981 cataacaaag ccatcaaacc ggacgccaga gattccgcgc ctgttgcgca tggcttcgcc
     5041 attttatgcg caataggcgc gccaccctgt cgccgtttat ggcg
//
